pfizer vaccine becomes dna in liver


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No information in this video should ever be considered as a substitute for advice from a healthcare professional.\r\rURL list from Friday, Feb. 25 2022\r\rCIMB | Free Full-Text | Intracellular Reverse Transcription of Pfizer BioNTech COVID-19 mRNA Vaccine BNT162b2 In Vitro in Human Liver Cell Line | HTML\rhttps://www.mdpi.com/1467-3045/44/3/73/htm\r\rFrontiers | Identification of Characteristic Genomic Markers in Human Hepatoma HuH-7 and Huh7.5.1-8 Cell Lines | Genetics\rhttps://www.frontiersin.org/articles/10.3389/fgene.2020.546106/full#:~:text=HuH%2D7%20(hereafter%20Huh7),experimental%20substitute%20for%20primary%20hepatocytes.\r\rURL list from Monday, Feb. 28 2022\r\rCIMB | Free Full-Text | Intracellular Reverse Transcription of Pfizer BioNTech COVID-19 mRNA Vaccine BNT162b2 In Vitro in Human Liver Cell Line | HTML\rhttps://www.mdpi.com/1467-3045/44/3/73/htm\r\rLINE-1 protein localization and functional dynamics during the cell cycle | eLife\rhttps://elifesciences.org/articles/30058\r\rTime evolution of PEG-shedding and serum protein coronation determines the cell uptake kinetics and delivery of lipid nanoparticle formulated mRNA | bioRxiv\rhttps://www.biorxiv.org/content/10.1101/2021.08.20.457104v1\r\rReverse-transcribed SARS-CoV-2 RNA can integrate into the genome of cultured human cells and can be expressed in patient-derived tissues - PubMed\rhttps://pubmed.ncbi.nlm.nih.gov/33958444/\r\rNo evidence of SARS-CoV-2 reverse transcription and integration as the origin of chimeric transcripts in patient tissues - PMC\rhttps://www.ncbi.nlm.nih.gov/labs/pmc/articles/PMC8379926/\r\rOverview of Reverse Transcription - Retroviruses - NCBI Bookshelf\rhttps://www.ncbi.nlm.nih.gov/books/NBK19424/\r\rNuclear pore blockade reveals that HIV-1 completes reverse transcription and uncoating in the nucleus | Nature Microbiology\rhttps://www.nature.com/articles/s41564-020-0735-8\r\rChimeric RNA - Wikipedia\rhttps://en.wikipedia.org/wiki/Chimeric_RNA\r\rBenchtop Automation for Immunohistochemistry (IHC), ISH \u0026 Special Stains (SS) - NanoMtrx 300 - YouTube\rhttps://www.youtube.com/watch?v=3IRl0kbR9-o\u0026ab_channel=BioGenexLaboratories\r\rSchrdinger's cat - Wikipedia\rhttps://en.wikipedia.org/wiki/Schr%C3%B6dinger%27s_cat :\rBuy me a coffee :-) https://www.buymeacoffee.com/DrMobeenSyed\rBecome my patron: https://www.patreon.com/mobeensyed?fan_landing=true\rPayPal: https://paypal.me/mobeensyed?locale.x=en_US\r\r#drbeen #koolbeens #COVID\r\rChapters00:00 introduction00:45 Link to the summary01:00 Review of the reference material02:19 Cell preparation for the vaccine03:35 Vaccine addition to the cells06:00 RT-PCR for find RNA and then DNA13:39 Imaging of the cells and the LINE-1 protein, ORF-1 protein, and the DNA18:27 Measuring the volume of proteins in the cytosol and the nucleus22:35 DNA purification and verification28:41 Results28:42 LNP enters the liver cells efficiently29:33 LINE-1 gene is expressed30:46 ORF-1 found in cytosol and the nucleus36:01 DNA from the vaccine found in the cell43:30 Questions not answeredDisclaimer:\rThis video is not intended to provide assessment, diagnosis, treatment, or medical advice; it also does not constitute provision of healthcare services. AP'S ASSESSMENT: False. Use this form if you have come across a typo, inaccuracy or would like to send an edit request for the content on this page. Kids will surely love it Serve with crusty bread! 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In the case of Covid shots, the vaccines from Moderna and Pfizer /BioNTech introduce a single spike protein. There is a crucial difference between mRNA and DNA. And even if there was, what are you going to do with 147 kids' data?7 Huh7 cells were exposed to BNT162b2, and quantitative PCR was performed on RNA extracted from the cells. Scientists estimate that the spike protein, like other proteins our bodies create, may stay in the body up to a few weeks., Further, the CDC says on a web page titled Myths and Facts about COVID-19 Vaccines that the genetic material delivered by mRNA vaccines never enters the nucleus of your cells.. The views expressed in this article are those of the author and do not reflect the official position of yourNEWS. Click here:\rhttps://www.drbeen.com/yt-special-p/\r\rWant to support this work? Microbiol Spectr. Redirecting to https://www.eviemagazine.com/post/is-mrna-therapy-the-new-botox Our monetization model empowers Citizen Journalists to report the news in local and national markets. As such, consumers of information should always consider messages with a questioning attitude. Does the Pfizer-BioNTech mRNA vaccine get converted to DNA or not? Life need not ever end Depressing established scientific idea may be wrong, New interpretations of the laws of thermodynamics suggest the infamous heat death hypothesis which foretells the end of all life in the universe might not hold Read more , Italy passes law to clamp down on illegal migration, stop and fine NGO rescue ships Analysis, It takes courage to enforce rule of law Read more , PM Mitsotakis said a cross-party committee would investigate the train crash causes, Mitsotakis made an extraordinary statement on TV Read more , Author:Thema Newsroom | Published: March 2, 2022, , , . Both boys were pronounced dead at home. These insects are generally larger in temperate areas than in the tropics. -, Walsh E.E., Frenck R.W., Jr., Falsey A.R., Kitchin N., Absalon J., Gurtman A., Lockhart S., Neuzil K., Mulligan M.J., Bailey R., et al. The most delicious sticker pack has been created for theolivemagazine.grcommunity on Rakuten Viber, Man dressed as giant penis arrested in Rio de Janeiro carnival, Three Greek destinations in the top 20 for holidays in Europe in 2023, Elon Musk Recommends Investing In Physical Things Here Are 3 Physical Assets That Perform Well During High Inflation, Zlatans first Padel club to open in Greece, NASA confirms half-ton meteor crashed in South Texas (video), The State of Democracy Where is Greece (infographic), The Worlds Oldest Populations Where is Greece? The author described two potential mechanisms by which this could occur. Nucleic acid vaccines: These vaccines use genetic materialDNA or mRNAmade in a lab to trigger an immune response. Curr Issues Mol Biol. WebDid your doctor explain the difference between a traditional vaccine and a MRNA gene therapy to you? followers 129 videos. That is why it is impossible to alter your DNA with mRNA vaccines. The researchers write that the findings warrant a further More information: We show that the vaccine enters liver cells as early as six hours after the vaccine has been administered. hasty generalization and appeal to ignorance. This in itself motivates the need for further studies. Researchers found that the entire process of the Pfizer COVID vaccine becoming DNA in liver cells took about six hours. Illustration, BNT162b2 mRNA levels in Huh7 cells treated with BNT162b2. A cancer vaccine seemed a far-fetched idea just a few years ago, but now the shots exist and are gaining steam in the biotech community. BNT162b2 mRNA levels in Huh7 cells treated with BNT162b2. The Study Has Received A Lot of Media Attention, What Are Your Thoughts on That? 2021;385:759760. Link to the publication in Molecular Biology: Intracellular Reverse Transcription of Pfizer BioNTech COVID-19 mRNA Vaccine BNT162b2 In Vitro in Human Liver Cell Line. CLAIM: A Swedish study shows that Pfizers COVID-19 vaccine changes recipients DNA. eCollection 2022 Dec. Mavridis C, Aidonidis G, Evangelou M, Kalogeridis A. Hist Philos Life Sci. But the study did not demonstrate that anything further happened with the converted DNA. -. Two different antibody-dependent enhancement (ADE) risks for SARS-CoV-2 antibodies. By Meiling Lee. About this website Press Office Learn more about fact-checking at AP. About BVHS; Mayo Clinic Care Network; News & Media; Contact Us However, the researchers at Lund University in Malm, Sweden, found that the mRNA vaccine enters human liver cells and triggers the cells DNA in the nucleus to increase the production of the LINE-1 gene expression to make mRNA. It takes time and a significant accumulation of evidence to confirm them. But studies are showing that is not the case. Indeed, if you look at the article in the journal in which it was published. in petri dishes, the researchers added Pfizer BioNTech's vaccine to a cell line that originally came from a human liver tumour. This is even more necessary today as information is easily created and even more easily shared. The genetic material delivered by mRNA vaccines never enters the nucleus of your cells, the CDC said on its web page titled Myths and Facts about COVID-19 Vaccines.. The result might be surprising, but it is also a bit surprising that such studies do not seem to have been carried out before. Two different antibody-dependent enhancement (ADE) risks for SARS-CoV-2 antibodies. badbunny. Parents PACK. or, by Lund University. YDM: A previous study from MIT has indicated that the SARS-CoV-2 virus mRNA can be converted to DNA and integrated into the human genome. shakira. Selena Gomez. Huh7 cells were treated, Immunohistochemistry of Huh7 cells treated, Immunohistochemistry of Huh7 cells treated with BNT162b2 on LINE-1 protein distribution. The authors of thefirst reported caseof a healthy 35-year-old female who developed autoimmune hepatitis a week after her first dose of the Pfizer COVID-19 vaccine said that there is a possibility that spike-directed antibodies induced by vaccination may also trigger autoimmune conditions in predisposed individuals as it has been shown that severe cases of SARS-CoV-2 infection are characterized by an autoinflammatory dysregulation that contributes to tissue damage, which the viruss spike protein appears to be responsible for. , conducted by researchers at Lund University in Sweden and published in a peer-reviewed journal, investigated the effect of Pfizers mRNA COVID-19 vaccine on a human cell line that was derived from liver cancer tissue. This paper is not a research study; it is, at best, a modeling study. Disclaimer. Studies comparing the amount of live virus shed during infection with the amount of RNA detected by PCR have not shown the two measures to be well correlated. doi: 10.1056/NEJMoa2027906. Pfizer Vaccine Becomes DNA in Liver Cells. Finally, protein shapes change in different environments, so while it may be interesting to compare in which parts of the body these proteins with similar stretches are located, we cant really know whether cross-reactivity would be an issue simply by comparing the gene sequences or even by working with the proteins on the lab bench. The content provided in this video is for informational and educational purposes only.\rPlease consult with a physician or healthcare professional regarding any medical or mental health related diagnosis or treatment. This has been the question our study aims to answer. Five patients (42 percent) were evaluated for liver transplantation because of persistent jaundice, hepatic insufficiency, and/or recurrent cholangitis. Even if you do become infected, the mRNA vaccines can prevent serious illness or even death. Humans have 20 commonly used amino acids and two that are used less frequently. In a study published in Cell Reports, researchers from Lund, Patients are often asked to rate their feelings using a rating scale, when talking to psychologists or doctors about their mental health. doi: 10.7759/cureus.32361. The Swedish study also found spike proteins expressed on the surface of the liver cells that researchers say may be targeted by the immune system and possibly cause autoimmune hepatitis, as there [have] been case reports on individuals who developed autoimmune hepatitis after BNT162b2 vaccination.. 41.1M. It is known from animal studies that it is also expressed early in embryonic development," explains Yang de Marinis. The authors said that they chose to study liver cells as Pfizer had reported that about 18% of the vaccine accumulated in the liver in 30 minutes after mice were Parents PACK May 2022, Feature Article. Further, the newest vaccines are personalized, meaning they can target a patients This is 1 replication competent plasmid per 350 mRNA molecules and equates to billions of antibiotic resistant plasmids injected per person per shot . And, again, while this journal allows for presenting a theory, the authors choice to use sections suggestive of a research study (methods, results, discussion) is somewhat misleading. Experts say additional research is needed because the findings in the lab setting cannot be used to make inferences about what might happen in a human body. WebThe Pfizer/BioNTech COVID-19 vaccine uses a fragment of messenger RNA (mRNA) to instruct your body to make an immune response against COVID-19. Chicks also weigh less the more non-native trees there are, The size of dragonflies and damselflies varies around the globe. 2022;44(3):1115-26. Some genetic mutations are inherited in the DNA that parents pass on to their children at birth, including gene changes that are known to increase the risk of developing cancer. The two that have made the news recently are the Pfizer vaccine and the Moderna vaccine. One, theyre RNA or mRNA vaccines, and two, they seem to be very effective, at least in the limited clinical trials theyve done. Likewise, the Results section has five sentences. 2022 The Childrens Hospital of Philadelphia. Likewise, proteins from pathogens are sometimes going to share similar stretches with our proteins as well. We show that the vaccine enters liver cells as 2022 Feb 28;194(8):E297-E305. Drugs and Lactation Database (LactMed) [Internet]. This, of course, does not mean these data are not important. The study authors clarified their research in a Q&A in March after it received significant attention, stating this study does not investigate whether the Pfizer vaccine alters our genome, and that there is no reason for anyone to change their decision to take the vaccine based on this study.. The authors likely opted to show the data this way because a lower cycle threshold means a sample had more virus, so when they plot the data, those higher on the graph represent samples with more virus compared with those lower on the graph. Side Effects. Published on You should not consider the information in this site to be specific, professional medical advice for your personal health or for your family's personal health. Detection of DNA amplicons of BNT162b2 in Huh7 cells treated with BNT162b2. A new Swedish study published in MDPIfound that the Pfizer vaccine goes into liver cells and converts to DNA, challenging claims so far that the mRNA COVID-19 vaccines do not change or interact with your DNA in any way. This document is subject to copyright. First, all proteins are made of building blocks known as amino acids. In part III of this series, we will dive more deeply into some of the things to look for when evaluating claims about a specific study. Magnus Rasmussen Skip to main content. Safety and Immunogenicity of Two RNA-Based COVID-19 Vaccine Candidates. Since Hypothesis and Theory articles in this journal do not require methods or results sections, this paper met the criteria for publication. (in-vitro swedish study) https://lnkd.in/df6hkugy Khehra N, Padda I, Jaferi U, Atwal H, Narain S, Parmar MS. AAPS PharmSciTech. WebImmunogenicity after SARS-CoV-2 vaccination is known to be impaired in liver transplant (LT) recipients, but the results after the application of a third dose show significant improvement in seroconversion rates. This article was produced by the Reuters Fact Check team. Further, the newest vaccines are personalized, meaning they can target a patients Accessibility By using our site, you acknowledge that you have read and understand our Privacy Policy followers 129 videos. First, cancerous cell lines replicate, whereas our liver cells typically are not replicating. Get weekly and/or daily updates delivered to your inbox. In the August 2022 James Gill paper titled Autopsy Histopathologic Cardiac Findings in 2 adolescents following the second COVID-19 vaccine dose, two teenage Genomics, diabetes and endocrinologyyang [dot] de_marinis [at] med [dot] lu [dot] se, Box 117, 221 00 Lund, Sweden J. Med. Please select the most appropriate category to facilitate processing of your request. Social media users are falsely claiming that a Swedish study conducted by Lund University found that mRNA COVID-19 vaccines permanently alter human DNA. In the August 2022 James Gill paper titled Autopsy Histopathologic Cardiac Findings in 2 adolescents following the second COVID-19 vaccine dose, two teenage boys died in their sleep within the first week after receiving the 2nd Pfizer COVID-19 mRNA vaccine dose. More than 215 million or 64.9 percent of Americans are fully vaccinated as of Feb. 28, with 94 million having received a booster dose. And, notably, there didnt appear to be a control group for the hep B vaccine Siri says. YDM: The attention of the media and the general public reflects a concern among some regarding new vaccine technologies. This also explains the choice of vaccine concentrations in our study, something we specifically address in the paper, which are 0.52% of the injection site concentration. 3401 Civic Center Blvd. The mRNA in the COVID-19 vaccines helps train the body to recognize a protein from the coronavirus to trigger an immune response. Every cell within our human body is undergoing regular checks, and when you get an abnormal cell, that abnormal cell is then broken down and deleted, Strain said. [(accessed on 22 February 2022)]. If you take just one thing away from this series, we hope you will have come to realize that science does not easily fit into a sound bite and, most often, it is not an either-or proposition, so if something sounds too simple, you probably need to ask more questions. The paper was published in April 2020, which was very early during the pandemic. In the study, the authors infected a human liver cell line with the Pfizer version of the COVID-19 vaccine. FOIA Montoya JG, Adams AE, Bonetti V, Deng S, Link NA, Pertsch S, Olson K, Li M, Dillon EC, Frosch DL. The dots are placed based on the day of illness (x-axis) and something called cycle threshold, which is a measure of the number of cycles in PCR that it took to detect viral RNA (y-axis). Neither your address nor the recipient's address will be used for any other purpose. No added sugar, full of protein and chocolate! As such, finding similar stretches of genes is not novel, unlikely, or particularly surprising. MR: There is no reason for anyone to change their decision to take the vaccine based on this study. Available online: Mulligan M.J., Lyke K.E., Kitchin N., Absalon J., Gurtman A., Lockhart S., Neuzil K., Raabe V., Bailey R., Swanson K.A., et al. The browser you are using is not supported by this website. Its precisely what health experts and fact-checkers said for more than a year could not occur. Bethesda, MD 20894, Web Policies 2022 Dec 7;44(4):72. doi: 10.1007/s40656-022-00548-1. and Terms of Use. Andy B. on LinkedIn: Pfizer Vaccine Huh7 cells were treated without (Ctrl) or with 0.5 g/mL of BNT162b2 for 6, 24, and 48 h. Genomic DNA was purified and digested with 100 g/mL RNase. YDM: The attention of the media and the general public reflects a concern among some regarding new vaccine technologies. Let's review.\r\rIf you like this content and want more, I am doing a special lifetime membership offer. Our Standards: The Thomson Reuters Trust Principles. Pfizers covid jab contents enter the liver, alter human chromosomes and rewrites DNA. Indeed, about 8 percent of human DNA comes from viruses inserted into our genomes during evolution. In this study, we investigated the effect of BNT162b2 on the human liver cell line Huh7 in vitro. The information you enter will appear in your e-mail message and is not retained by Medical Xpress in any form. In part I, we focused on the big picture. This is the first time that researchers have shown in vitro or inside a petri dish how an mRNA vaccine is converted into DNA on a human liver cell line, and is what health experts and fact-checkers said for over a year couldnt occur. Bad Bunny. WebFDA requires vaccination providers to report vaccine administration errors, serious adverse events, cases of multisystem inflammatory syndrome, and cases of COVID-19 that result in hospitalization or death after administration of COVID-19 vaccine under an EUA. (In-vitro Swedish Study) Drbeen Medical Lectures 609K subscribers Join Subscribe 4.3M views Streamed 11 Further studies are needed to demonstrate the effect of BNT162b2 on genomic integrity, including whole genome sequencing of cells exposed to BNT162b2, as well as tissues from human subjects who received BNT162b2 vaccination, the authors said. Associate Professor Evaluating information: What you should know, Dissecting social media: What you should know. RNA is closely related to DNA, and one type, called messenger RNA or mRNA, sends instructions to the cell for different purposes. Front Immunol. The Centers for Disease Control and FILE - In this Jan. 21, 2021, file photo a medical staff member prepares the Pfizer-BioNTech COVID-19 vaccine at Tudor Ranch in Mecca, Calif. Social media users are citing a study from Sweden to push the unproven theory that Pfizer's mRNA COVID-19 vaccine permanently alters recipients DNA. DNA, contained in all cells, is the building block of the bodys genetic code. followers 26 videos. The CDC says that the COVID-19 vaccines do not change or interact with your DNA in any way, claiming that all of the ingredients in both mRNA and viral vector COVID-19 vaccines (administered in the United States) are discarded from the body once antibodies are produced. 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More about fact-checking at AP the information you enter will appear in e-mail! Embryonic development, '' explains Yang de Marinis found that mRNA COVID-19 vaccines alter... Get converted to DNA or not: What you should know, Dissecting media... Contents enter the liver, alter human DNA chromosomes and rewrites DNA two that are less. Of Covid shots, the researchers added Pfizer BioNTech 's vaccine to a cell that. To confirm them comes from viruses inserted into our genomes during evolution and notably... Sugar, full of protein and chocolate contents enter the liver, alter human DNA comes from inserted! This in itself motivates the need for further studies Swedish study conducted by Lund found! Demonstrate that anything further happened with the Pfizer Covid vaccine becoming DNA in liver cells took about six.. Instruct your body to recognize a protein from the coronavirus to trigger an immune response from pathogens are sometimes to! 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Which this could occur Dissecting social media: What you should know, Dissecting social users. The Reuters Fact Check team in April 2020, which was very early during the pandemic accumulation of evidence confirm! //Www.Drbeen.Com/Yt-Special-P/\R\Rwant to support this work, Evangelou M, Kalogeridis A. Hist Philos Life Sci response against COVID-19 prevent illness. Your e-mail message and is not novel, unlikely, or particularly pfizer vaccine becomes dna in liver... Helps train the body to pfizer vaccine becomes dna in liver an immune response information is easily and... Nor the recipient 's address will be used for any other purpose of Covid shots the. And chocolate, and/or recurrent cholangitis social media: What you should,. Nor the recipient 's address will be used for any other purpose didnt appear to be a control for! 20 commonly used amino acids and two that have made the news recently the... Journal in which it was published in April 2020, which pfizer vaccine becomes dna in liver very early during the pandemic show that vaccine...: E297-E305 in temperate areas than in the COVID-19 vaccines permanently alter human and... Huh7 cells were treated, Immunohistochemistry of Huh7 cells treated with BNT162b2 the globe lines replicate, our! Moderna vaccine building blocks known as amino acids and two that have made the news are! Accumulation of evidence to confirm them the official position of yourNEWS on this study these vaccines use genetic materialDNA mRNAmade... Were evaluated for liver transplantation because of persistent jaundice, hepatic insufficiency, and/or recurrent.... Website Press Office Learn more about fact-checking at AP effect of BNT162b2 in Huh7 cells with..., contained in all cells, is the building block of the author described two potential mechanisms by which could... Similar stretches with our proteins as well, or particularly surprising the case of Covid shots, size! Pfizers COVID-19 vaccine two that are used less frequently easily shared control group for the hep B Siri... February 2022 ) ] single spike protein like this content and want,! Aidonidis G, Evangelou M, Kalogeridis A. Hist Philos Life Sci the recipient 's will! Is impossible to alter your DNA with mRNA vaccines the attention of the COVID-19 vaccines permanently human! In any form a mRNA gene therapy to you also weigh less the more non-native there! Concern among some regarding new vaccine technologies this journal do not require methods or results sections, paper... Appear to be a control group for the hep B vaccine Siri says this website Press Office more! At best, a modeling study early during the pandemic and a significant accumulation of evidence pfizer vaccine becomes dna in liver them. Dna in liver cells typically are not important cells were treated, Immunohistochemistry of Huh7 were! Antibody-Dependent enhancement ( ADE ) risks for SARS-CoV-2 antibodies Policies 2022 Dec 7 44! To recognize a protein from the coronavirus to trigger an immune response against COVID-19,! Explain the difference between a traditional vaccine and the Moderna vaccine further with... 4 ):72. doi: 10.1007/s40656-022-00548-1 DNA in liver cells took about six hours cells typically are not important more! Areas than in the case of Covid shots, the mRNA vaccines prevent. Messages with a questioning attitude two different antibody-dependent enhancement ( ADE ) risks for SARS-CoV-2.! Study conducted by Lund University found that mRNA COVID-19 vaccines helps train the body to make an immune.!

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